SE195:/S1411

Sample Set Information

ID	SE195
Title	LC-MS analysis of LIPID REMODELLING REGULATOR 1 (LRL1) transgenic lines of Chlamydomonas reinhardtii
Description	A metabolome analysis was conducted by LC-MS using a wild type Chlamydomonas reinhardtii (C9) and a paromomycin insertional LIPID REMODELLING REGULATOR 1 (LRL1) mutant line (lrl1-1) grown in phosphorus (P)-replete and P-deplete conditions for 2, 4 and 8 days.
Authors	Nur Akmalia Hidayati, Yui Yamada-Oshima, Masako Iwai, Takashi Yamano, Masataka Kajikawa, Nozomu Sakurai, Kunihiro Suda, Kanami Sesoko, Koichi Hori, Takeshi Obayashi, Mie Shimojima, Hideya Fukuzawa, Hiroyuki Ohta
Reference	LIPID REMODELLING REGULATOR 1 (LRL1) is differently involved in the phosphorus-depletion response from PSR1 in Chlamydomonas reinhardtii. The Plant Journal (in press)
Comment

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Sample Information

ID	S1411
Title	TAP, day4, lrl1-1, rep1
Organism - Scientific Name	Chlamydomonas reinhardtii
Organism - ID	NCBI Taxonomy:3055
Compound - ID
Compound - Source
Preparation	Mutant strain lrl1-1 was cultured in TAP medium for 4 days.
Sample Preparation Details ID	SS1
Comment

Sample Preparation Details Information

ID	SS1
Title	C. reinhardtii strains and culture conditions
Description	The C. reinhardtii strain C9 (CC-408, wild type mt-) and lrl1-1 were obtained from the Kyoto University C. reinhardtii mutant library. Screening of lrl1-1 from the Chlamydomonas mutant library was performed according to Gonzalez-Ballestar et al. 2014 . Briefly, several primers spanning the upstream region to the 3' end region of Cre03.g197100 were designed. The superpool library was used as the PCR template, and any amplified product from a primer pair that included a specific region of Cre03.g197100 and AphVIII were sequenced, and its positional tag was identified. Further DNA sequencing indicated that AphVIII-tag insertion was in the upstream region of Cre03.g197100, 115 bp upstream of the 5' untranslated region (UTR), which is predicted to be the promoter region. Liquid cultures were grown in an Erlenmeyer flask mixotrophically in Tris-acetate-phosphate (TAP) medium. For all cultures, cells were cultivated under continuous illumination at 20-40 umol m^-2 s^-1 and 100 rpm shaking at 25C. To induce P-starvation, mid-log phase cells (3-5 x10^6 cells/ml) were centrifuged at 2000 rpm for 5 min and washed twice in a P-free (TAP-P) medium. Potassium phosphate was replaced by 1.5 mM KCl in TAP-P medium. Cells were initially adjusted to 1x10^5 cells/ml on culture day 0. Cultured cells were collected by centrifugation, immediately frozen in liquid nitrogen, and stored at -80C until use.
Comment_of_details