SE207:/S012/M01
Sample Set Information
| ID | TSUMURA02 |
|---|---|
| Title | Non-targeted metabolome analysis of Japanese kampo medicine, maoto (ma huang tang) and plasma from maoto-administered human and rats by LC-Orbitrap LTQ MS |
| Description | The data contains the results of metabolic profiling of Japanese Kampo medicine maoto, plasma from maoto-administered human and rats using LC-LTQ Orbitrap XL-MS system (positive and negative mode) |
| Authors | Katsuya Ohbuchi |
| Reference | Ohbuchi, K., Sakurai, N., Kitagawa, H., Sato, M., Suzuki, H., Kushida, H., et al. (2020). Metabolomics, 16(5), 1–12. |
| Comment |
Sample Information
| ID | S012 |
|---|---|
| Title | Human plasma, 0.5h (sub. 1) |
| Organism - Scientific Name | Homo sapiens |
| Organism - ID | NCBI taxonomy 9606 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Human plasma was collected at 0.5 hours after maoto administration. |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M01 |
|---|---|
| Title | LC-MS, ESI, Positive |
| Method Details ID | MS01 |
| Sample Amount | |
| Comment |
Analytical Method Details Information
| ID | MS01 |
|---|---|
| Title | LC-Orbitrap, ESI, Positive |
| Instrument | Agilent1200 HPLC (Agilent), LTQ Orbitrap XL (Thermo Fisher Scientific) |
| Instrument Type | LC-Orbitrap-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | One hundred microliters of plasma sample were extracted with 300 uL of methanol or 1 mL of 75% MeOH (aq). The mixture was mixed and centrifuged. The supernatant was collected for analysis.
LC-MS was performed using an Agilent 1200 HPLC system (Agilent Technologies, Santa Clara, CA) coupled to an LTQ Orbitrap XL-MS system (Thermo Fisher Scientific, Inc., San Jose, CA), equipped with an electrospray source operating in either positive- or negative-ion modes. The spray voltage and capillary temperature were 4 kV and 300°C, respectively. The analysis consisted of 2 scan events. Scan event 1 was a full mass type (Analyzer, FTMS; Resolution, 60,000). Scan event 2 was an MS/MS type (analyzer, Ion Trap MS; act type, collision-induced dissociation; normalized collision energy, 35.0%). An aliquot of the extracted sample (5 µL) was injected into a TSK gel ODS-100V reversed-phase column (column size, 3.0 × 50 mm; particle size, 5.0 µm; Tosoh Corp., Tokyo, Japan). The column temperature was set at 40°C. Mobile phases A (0.1% formic acid) and B (acetonitrile with 0.1% formic acid) were used with a gradient of 3% to 97% B from 0 to 15 min, 97% B from 15 to 20 min, 97% to 3% B from 20 to 20.1 min, and 3% B for 4.9 min before the next injection, at a flow rate of 400 µL/min. Data were acquired with Xcalibur software (Thermo Fisher Scientific Inc., San Jose, CA). |
| Comment_of_details |
