SE229:/S230/M11
From Metabolonote
Sample Set Information
| ID | SE229 |
|---|---|
| Title | LC-MS based untargeted metabolome analysis of Oryza |
| Description | Compounds in the Oryza species were analyzed using liquid chromatography-mass spectrometry (LC-MS) in a untargeted manner. The same analytical conditions are applied to all samples. Therefore, the compound peaks can be compared to each other by the mass values, the retention time of the LC, and the CID mass spectrum. |
| Authors | Yutaka Sato (National Institute of Genetics), Nozomu Sakurai (National Institute of Genetics, Kazusa DNA Research Institute, email: sakurai (at) kazusa.or.jp) |
| Reference | |
| Comment |
Sample Information
| ID | S230 |
|---|---|
| Title | Rice seed, O. sativa, WRC55 (No.179-1) |
| Organism - Scientific Name | Oryza sativa |
| Organism - ID | NCBI taxonomy: 4530 |
| Compound - ID | |
| Compound - Source | |
| Preparation | The sample was provided by the Oryzabase sponsored by NBRP. |
| Sample Preparation Details ID | SS1 |
| Comment |
Sample Preparation Details Information
| ID | SS1 |
|---|---|
| Title | Sample preparation |
| Description | The harvested sample was stored at low temperature. The sample was ground to a fine powder using a homogenizer Multi-beads shocker MB3200(S) (Yasui Kikai Corp., Osaka, Japan) with 3,000 rpm for 15 min and stored at -80 degree C until use. |
| Comment_of_details |
Analytical Method Information
| ID | M11 |
|---|---|
| Title | LC-QTOF-MS, ESI, Negative |
| Method Details ID | MS11 |
| Sample Amount | 0.25 mg / 2 ul injection |
| Comment |
Analytical Method Details Information
| ID | MS11 |
|---|---|
| Title | LC-Q-Tof-MS, ESI, Negative |
| Instrument | Nexera X2 (Shimadzu), Compact (Bruker Daltonics) |
| Instrument Type | LC-QTOF-MS |
| Ionization | ESI |
| Ion Mode | Negative |
| Description | Compound extraction and LC-MS measurement were performed as same as described in the ESI positive mode (MS01, http://metabolonote.kazusa.or.jp/SE229:/MS01) except for modifications below: polarity, negative; MS/MS Collision energy. |
| Comment_of_details | [column] InertSustain AQ-C18 (1.5 x 50 mm, 3 micrometer; GL Sciences)
[gradient] Solvent A: water containing 0.1% v/v formic acid; Solvent B: acetonitrile; Gradient 2% B (0 min), 2% B (1.5 min), 98% B (13.5 min), 98% B (17 min), 2% B (17.01 min), and 2% B (18.5 min) [total separation time] 18.5 min |
