SE40:/S23/M01
From Metabolonote
lower pageD01
Sample Set Information
| ID | SE40 |
|---|---|
| Title | Comparison of fruit metabolites among tomato cultivars |
| Description | Investigation of Tomato fruits metabolites. 28 cultivers and 3 replicates data are examined. |
| Authors | Takeshi Ara 1, Shingo Takahashi 1,3, Naoko Waki 1,3, Nozomu Sakurai 2, Haruya Takahashi 1, Shinsuke Mohri 1, Kunimi Suda 2, Keishi Ozawa 2, Takisawa Rihito 4, Akira Kitajima 4, Yasuki Matsumura 1, Teruo Kawada 1, Daisuke Shibata 1, 2, 1:Graduate School of Agriculture, Kyoto University, Japan, 2:Kazusa DNA Research Institute, Kazusa Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan, 3:Kagome Co., Ltd., Japan, 4:Experimental Farm of Kyoto University, Japan |
| Reference | Ara T. et al. (2021) Plant Direct 5(4) e00318 |
| Comment | version 1 |
TOMATOMET: http://metabolites.in/tomato-fruits/
PGDBj: http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t4081Sample Information
| ID | S23 |
|---|---|
| Title | Solanum lycopersicum KTF020 fruit |
| Organism - Scientific Name | Solanum lycopersicum |
| Organism - ID | NCBI taxonomy:4081 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Solanum lycopersicum KTF020 are grown in greenhouses of Kagome Co., Ltd. from summer 2013 to spring 2014. |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M01 |
|---|---|
| Title | LC-Orbitrap-MS, ESI Positive analysis |
| Method Details ID | MS1 |
| Sample Amount | |
| Comment | [MassBase ID] MDLC1_46888 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LC-Orbitrap-MS ESI positive method 1 |
| Instrument | Agilent1200 HPLC (Agilent), LTQ-Orbitrap (Thermo Fisher Scientific) |
| Instrument Type | LC-Orbitrap-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 5uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1200 series (Agilent), Column: TSKgel ODS-100V (3 x 50 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B) 0 to 3% for 15 min, 3 to 97% for 20 min, 97 to 3% for 0.1 min, and 3% for 25 min., Column temp.: 40 degree C, Flow rate=0.4mL/min, PDA: 190-950 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS + c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1). |
| Comment_of_details |
