SE40:/S26/M03/D01
From Metabolonote
Sample Set Information
| ID | SE40 |
|---|---|
| Title | Comparison of fruit metabolites among tomato cultivars |
| Description | Investigation of Tomato fruits metabolites. 28 cultivers and 3 replicates data are examined. |
| Authors | Takeshi Ara 1, Shingo Takahashi 1,3, Naoko Waki 1,3, Nozomu Sakurai 2, Haruya Takahashi 1, Shinsuke Mohri 1, Kunimi Suda 2, Keishi Ozawa 2, Takisawa Rihito 4, Akira Kitajima 4, Yasuki Matsumura 1, Teruo Kawada 1, Daisuke Shibata 1, 2, 1:Graduate School of Agriculture, Kyoto University, Japan, 2:Kazusa DNA Research Institute, Kazusa Kamatari 2-6-7, Kisarazu, Chiba 292-0818, Japan, 3:Kagome Co., Ltd., Japan, 4:Experimental Farm of Kyoto University, Japan |
| Reference | Ara T. et al. (2021) Plant Direct 5(4) e00318 |
| Comment | version 1 |
TOMATOMET: http://metabolites.in/tomato-fruits/
PGDBj: http://pgdbj.jp/plantdb/plantinfo.html?ln=en&cmd=entry&ppid=t4081Sample Information
| ID | S26 |
|---|---|
| Title | Solanum lycopersicum Sicilian Rouge fruit |
| Organism - Scientific Name | Solanum lycopersicum |
| Organism - ID | NCBI taxonomy:4081 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Solanum lycopersicum Sicilian Rouge were purchased at a local market of Kyoto city, (Kyoto, Japan) (November, 2015). |
| Sample Preparation Details ID | |
| Comment | Kyoto-san. |
Analytical Method Information
| ID | M03 |
|---|---|
| Title | LC-Orbitrap-MS, ESI Positive analysis |
| Method Details ID | MS2 |
| Sample Amount | |
| Comment | [MassBase ID] MDLC1_47209 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS2 |
|---|---|
| Title | LC-Orbitrap-MS ESI positive method 2 |
| Instrument | Nexera HPLC (Shimadzu), LTQ-Orbitrap Velos Pro (Thermo Fisher Scientific) |
| Instrument Type | LC-Orbitrap-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 5uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Shimadzu Nexera (Shimadzu), Column: TSKgel ODS-100V (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B) 0 to 3% for 15 min, 3 to 97% for 20 min, 97 to 3% for 0.1 min, and 3% for 25 min., Column temp.: 40 degree C, Flow rate=0.4mL/min, PDA: 190-800 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS + c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1). |
| Comment_of_details |
Data Analysis Information
| ID | D01 |
|---|---|
| Title | PowerGet data analysis |
| Data Analysis Details ID | DS1 |
| Recommended decimal places of m/z | 6|ITMS 2 |
| Comment |
Data Analysis Details Information
| ID | DS1 |
|---|---|
| Title | PowerGet analysis for detection of all peaks |
| Description | Raw data files are converted to text file by MSGet software without cut off value and peaks are extracted from the text files by PowerFT with parameters (intensity cut off=20000, margin of ion grouping in peak detection=5, peak selection filter is default, intensity cut off in peak assignment=1000). The replicates data are aligned by PowerMatch with blank data. The alignment is manually edited. Assigned peaks observed in multiple replicate samples are selected for annotation process. |
| Comment_of_details |
