SE41:/MS1

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Sample Set Information

ID	SE41
Title	Comparison of tomato fruit metabolites among fruit maturation
Description	Investigation of Tomato fruits metabolites. 2 cultivers, 4 deveropmental stages and 3 replicates data are examined.
Authors	Takeshi Ara 1, Shingo Takahashi 1,3, Naoko Waki 1,3, Nozomu Sakurai 2, Haruya Takahashi 1, Shinsuke Mohri 1, Kunihiro Suda 2, Keishi Ozawa 2, Koichi Aizawa 3, Hiroyuki Suganuma 3, Yasuki Matsumura 1, Teruo Kawada 1, Daisuke Shibata 1, 2, 1:Graduate School of Agriculture, Kyoto University, Japan, 2:Kazusa DNA Research Institute, Japan, 3:KAGOME CO., LTD., Japan
Reference	Ara T. et al. (2017) Acta Horticulturae 1159: 101-106.
Comment	version 1

ID	MS1
Title	LC-Orbitrap-MS ESI positive method
Instrument	Agilent1200 HPLC (Agilent), LTQ-Orbitrap (Thermo Fisher Scientific)
Instrument Type	LC-Orbitrap-MS
Ionization	ESI
Ion Mode	Positive
Description	Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 5uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1200 series (Agilent), Column: TSKgel ODS-100V (3 x 50 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B) 3 to 97% for 85 min, 97% for 10 min, 97 to 3% for 0.1 min, and 3% for 5 min., Column temp.: 40 degree C, Flow rate=0.4mL/min, PDA: 190-950 nm (2 nm step). Orbitrap-MS conditions: Filter 1: FTMS + c norm !corona !pi res=60000 o(100.0-1500.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm !corona !pi Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm !corona !pi Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm !corona !pi Dep MS/MS 4th most intense ion from (1).
Comment_of_details