SE44:/S02
From Metabolonote
Sample Set Information
| ID | SE44 |
|---|---|
| Title | Physcomitrella patens metabolite analysis using stable isotopes |
| Description | Metabolites in the Physcomitrella patens are investigated by using stable isotope labelings. This analysis was performed to estimate the elemental composition with high reliability using the number of stable isotopes incorporated in the compound as an index and to add information to discuss the biosynthetic pathway. The Physcomitrella patens were labeled with 13C, 15N, 18O, and 34S, respectively in the same condition. Following peak picking by using PowerGet, the metabolite list was prepared from unlabeled data and blank data. The isotopic peaks were searched and listed using ShiftedIonsFinder. Moreover, the flavonoid-like peaks were also searched using ShiftedIonsFinder. |
| Authors | Yasuhide Hiraga 1,2, Takeshi Ara 1, Yoshiki Nagashima 1, Nozomu Sakurai 1, Hideyuki Suzuki 1,2, Kota Kera 1,3; 1:Kazusa DNA Research Institute, Japan, 2:Hirata Corporation, Japan, 3:Faculty of Applied Bioscience, Tokyo University of Agriculture, Japan |
| Reference | Hiraga Y, et al, (2020) Plant Biotechnology, 37(3), 377-381. |
| Comment |
Sample Information
| ID | S02 |
|---|---|
| Title | Physcomitrella patens subsp. patens with 18O treatment |
| Organism - Scientific Name | Physcomitrella patens subsp. patens |
| Organism - ID | NCBI taxonomy:3218 |
| Compound - ID | |
| Compound - Source | |
| Preparation | |
| Sample Preparation Details ID | SS1 |
| Comment |
Sample Preparation Details Information
| ID | SS1 |
|---|---|
| Title | Sealed-glass bottle system |
| Description | The gametophore of P. patens were grown on solid (0.5 % phytagel) half strength Murashige-Skoog (MS) medium containing 0.5 % glucose at 22°C under 18 h-light/6 h-dark long day condition using sealed-glass bottle system for 2 months. Gametophores were harvested as three biological replicates, frozen in liquid nitrogen, and then lyophilized. |
| Comment_of_details |
