SE8:/S01/M02
From Metabolonote
Sample Set Information
| ID | SE8 |
|---|---|
| Title | Grifola frondosa metabolite analysis |
| Description | Investigation of Grifola frondosa metabolites. 4 replicates data are examined. |
| Authors | Takeshi Ara, Daisuke Nakajima, Mitsuo Enomoto, Nozomu Sakurai, Hideyuki Suzuki, Koei Okazaki, Daisuke Shibata, Kazusa DNA Research Institute |
| Reference | Direct Submittion |
| Comment | version 1 |
Sample Information
| ID | S01 |
|---|---|
| Title | Grifola frondosa |
| Organism - Scientific Name | Grifola frondosa |
| Organism - ID | NCBI taxonomy:5627 |
| Compound - ID | |
| Compound - Source | |
| Preparation | The Maitake mushroom samples are purchased at the local market. |
| Sample Preparation Details ID | |
| Comment | [KomicMarket ID] KSBA_29 |
Analytical Method Information
| ID | M02 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Method Details ID | MS2 |
| Sample Amount | 6.7mg |
| Comment | [MassBase ID] MDLC1_10287 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS2 |
|---|---|
| Title | LC-FT-ICR-MS ESI positive method 2 |
| Instrument | Agilent1100 HPLC (Agilent), LTQ-FT (Thermo Fisher Scientific) |
| Instrument Type | LC-FTICR-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Column: TSKgel-100Z (4.6 x 250 mm, 5 micrometer; TOSOH), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 30% (0.0 to 25.0 min), 30 to 90% (25.1 to 40.0 min), 95% (45.1 to 50.0 min), 3% (50.1 to 57.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=100000 o(50.0-2000.0); 2: ITMS + c norm !corona !pi Dep MS/MS Most intense ion from (1); 3: ITMS + c norm Dep MS/MS 2nd most intense ion from (1); 4: ITMS + c norm Dep MS/MS 3rd most intense ion from (1); 5: ITMS + c norm Dep MS/MS 4th most intense ion from (1); 6: ITMS + c norm Dep MS/MS 5th most intense ion from (1), Reject Mass List:224.1900, 249.2100, 266.2300, 294.2600, 554.1800, 756.5500, 782.5700, 783.5700, 804.5500 , 950.8100. |
| Comment_of_details |
