SE93:/S2/M3
From Metabolonote
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Sample Set Information
| ID | SE93 |
|---|---|
| Title | Analysis of Lotus japonicus |
| Description | Leaves of Lotus japonicus lines (MG-20 and B-129) were analyzed by LC-FT-ICR-MS. Two Arabidopsis thaliana samples were used as references. |
| Authors | Takeshi Ara, Hideyuki Suzuki, Daisuke Shibata, Nozomu Sakurai, Ryosuke Sasaki, Makoto Hirosawa, Lab. Genome Biotech, Kazusa DNA Research Institute, Japan; Center for Development of Biotech Industries, Kazusa DNA Research Institute, Japan |
| Reference | Direct submission |
| Comment | NS deposited the metadata, most of which were retrieved from MassBase. For more detailed information, please contact the authors directly or via NS (email: metabolonote [at] kazusa.or.jp). |
Sample Information
| ID | S2 |
|---|---|
| Title | Lotus japonicus MG-20 Leaf |
| Organism - Scientific Name | Lotus japonicus |
| Organism - ID | NCBI Taxonomy:34305 |
| Compound - ID | |
| Compound - Source | |
| Preparation | |
| Sample Preparation Details ID | |
| Comment |
Analytical Method Information
| ID | M3 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Method Details ID | MS1 |
| Sample Amount | |
| Comment | [MassBase ID] MDLC1_05769 |
The raw (binary) and near-raw (text) files of this analysis are available at MassBase.
Analytical Method Details Information
| ID | MS1 |
|---|---|
| Title | LC-FTICR-MS, ESI Positive analysis |
| Instrument | LC-FT-ICR-MS; [Agilent 1100 LC (Agilent Technologies); Finnigan LTQ-FT (Thermo Fisher Scientific K.K.)] |
| Instrument Type | LC-FTICR-MS |
| Ionization | ESI |
| Ion Mode | Positive |
| Description | Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 40% (0.0 to 23.0 min), 40 to 95% (23.0 to 23.1 min), 95% (23.1 to 25.0 min), 3% (25.1 to 30.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0), Rejected mass=266.0000;294.0000;391.0000. |
| Comment_of_details | [MassBase Method ID] MTLC0349 |
