Sample Set Information
| ID
|
SE93
|
| Title
|
Analysis of Lotus japonicus
|
| Description
|
Leaves of Lotus japonicus lines (MG-20 and B-129) were analyzed by LC-FT-ICR-MS. Two Arabidopsis thaliana samples were used as references.
|
| Authors
|
Takeshi Ara, Hideyuki Suzuki, Daisuke Shibata, Nozomu Sakurai, Ryosuke Sasaki, Makoto Hirosawa, Lab. Genome Biotech, Kazusa DNA Research Institute, Japan; Center for Development of Biotech Industries, Kazusa DNA Research Institute, Japan
|
| Reference
|
Direct submission
|
| Comment
|
NS deposited the metadata, most of which were retrieved from MassBase. For more detailed information, please contact the authors directly or via NS (email: metabolonote [at] kazusa.or.jp).
|
Sample Information
| ID
|
S3
|
| Title
|
Arabidopsis thaliana Col-0 Leaf
|
| Organism - Scientific Name
|
Arabidopsis thaliana
|
| Organism - ID
|
NCBI Taxonomy:3702
|
| Compound - ID
|
|
| Compound - Source
|
|
| Preparation
|
|
| Sample Preparation Details ID
|
|
| Comment
|
|
Analytical Method Details Information
| ID
|
MS1
|
| Title
|
LC-FTICR-MS, ESI Positive analysis
|
| Instrument
|
LC-FT-ICR-MS; [Agilent 1100 LC (Agilent Technologies); Finnigan LTQ-FT (Thermo Fisher Scientific K.K.)]
|
| Instrument Type
|
LC-FTICR-MS
|
| Ionization
|
ESI
|
| Ion Mode
|
Positive
|
| Description
|
Harvested sample is frozen by liquid N2 and resulting powder (100mg) are solved in 300uL 80% methanol solution. 20uL sample is injected into HPLC after 0.2um membrane filter treatment. HPLC conditions: Agilent 1100 series (Agilent), Solvent: A; 0.1% formic acid aq. B; ACN (addition 0.1% formic acid fc.), Gradient: (B);3 to 40% (0.0 to 23.0 min), 40 to 95% (23.0 to 23.1 min), 95% (23.1 to 25.0 min), 3% (25.1 to 30.0 min), Column temp.: 30 degree C, Flow rate=0.5mL/min, PDA: 200-650 nm (2 nm step). FT-ICR-MS conditions: Filter 1: FTMS + c norm !corona !pi res=50000 o(200.0-1500.0), Rejected mass=266.0000;294.0000;391.0000.
|
| Comment_of_details
|
[MassBase Method ID] MTLC0349
|
Data Analysis Information
| ID
|
D1
|
| Title
|
Auto peak detection by PowerGetBatch
|
| Data Analysis Details ID
|
DS1
|
| Recommended decimal places of m/z
|
|
| Comment
|
|
Data Analysis Details Information
| ID
|
DS1
|
| Title
|
Auto peak detection by PowerGetBatch
|
| Description
|
|
| Comment_of_details
|
|
