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Tetraploidization of A. thaliana was perfo … Tetraploidization of A. thaliana was performed in an accession Col-0, as described previously (Breuer et al. 2007), and the ploidy type was confirmed by flow cytometry analysis (Kozuka et al. 2005). Diploid and autotetraploid plants were maintained at 23 °C under continuous illumination (ca. 60 µmol/sec/m2) on rockwool supplied with 0.2 g/L HYPONeX solution, as described elsewhere (Fujikura et al. 2007). To harvest mature seeds for metabolomic analysis, 1-week-old plants grown on agar-solidified, half-strength Murashige and Skoog medium were transferred to soil [PRO-MIX (Premier Horticulture, Canada): vermiculite = 2:1] and grown under 16 h light/8 h dark with a nutrient solution reported as a control medium in Hirai et al. (1995). For biological replicates, seeds were independently collected from 12 individuals for each genotype (Supplementary Table S1). or each genotype (Supplementary Table S1).
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