SE125:/S1
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Sample Set Information
| ID | TSE4 |
|---|---|
| Title | A novel method for single-grain-based metabolic profiling of Arabidopsis seed |
| Description | In plant metabolomics, metabolite contents are often normalized by sample weight. However, accurate weighing of very small samples, such as individual Arabidopsis thaliana seeds (approximately 20 µg), is difficult, which may lead to irreproducible results. |
| Authors | Yuji Sawada, Hirokazu Tsukaya, Yimeng Li, Muneo Sato, Kensuke Kawade, Masami Yokota Hirai |
| Reference | Sawada et al. (2017) Metabolomics 13:75 |
| Comment |
The raw data files are available at DROP Met web site in PRIMe database of RIKEN.
Sample Information
| ID | S1 |
|---|---|
| Title | Arabidopsis thaliana seeds |
| Organism - Scientific Name | Arabidopsis thaliana |
| Organism - ID | NCBI taxonomy 3702 |
| Compound - ID | |
| Compound - Source | |
| Preparation | Tetraploidization of A. thaliana was performed in an accession Col-0, as described previously (Breuer et al. 2007), and the ploidy type was confirmed by flow cytometry analysis (Kozuka et al. 2005). Diploid and autotetraploid plants were maintained at 23 °C under continuous illumination (ca. 60 µmol/sec/m2) on rockwool supplied with 0.2 g/L HYPONeX solution, as described elsewhere (Fujikura et al. 2007). To harvest mature seeds for metabolomic analysis, 1-week-old plants grown on agar-solidified, half-strength Murashige and Skoog medium were transferred to soil [PRO-MIX (Premier Horticulture, Canada): vermiculite = 2:1] and grown under 16 h light/8 h dark with a nutrient solution reported as a control medium in Hirai et al. (1995). For biological replicates, seeds were independently collected from 12 individuals for each genotype (Supplementary Table S1). |
| Sample Preparation Details ID | |
| Comment |
