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The Arabidopsis mto mutants, mto1-1 (in ec … The Arabidopsis mto mutants, mto1-1 (in ecotype Columbia), mto1-4 (in Columbia), mto2 [in ecotype Wassilewskija (WS)], mto3-1 (in WS), and mto3-2 (in Columbia), have been described previously (Goto et al. 2005). Seedlings were grown on Murashige and Skoog medium (Wako, Osaka, Japan, #392-00591) with 0.8% agar, 1% sucrose at pH 5.8 under 16 h light/8 h dark cycles at 22°C for 18 days. Aerial parts were harvested from 4 to 10 biological replicates of each line (see Supplementary Data 1), 6 h after the onset of the light phase. The mto mutants were backcrossed ten times for mto1-1 and mto1-4, seven times for mto2, four times for mto3-1, and seven times for mto3-2 to the WT accession (Col-0) before phenotypic and metabolite profiling analysis. Two independent alleles (mto1-1 and mto1-4) for mto1, one (mto2-1) for mto2, and two (mto3-1 and mto3-2) for mto3 were used in this study. To minimize effects derived from the different harvesting periods, we compared mutants and the control plants that were harvested in the same period [hereafter called Col0_H1 (mto1), Col0_H2 (mto2), and Col0_H3 (mto3), Supplementary Fig. 1]. For metabolite profiling, these mutants and the corresponding WT were grown under strictly controlled conditions (Kusano et al. 2007). The mto2 plants exhibit dwarf phenotypes, whereas there are no visible phenotypic changes in the mto1 and mto3 mutants when compared with the WT (Fig. 1b). tants when compared with the WT (Fig. 1b).
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