SE139:/S1

Sample Set Information

ID	TSE1240
Title	Comparative metabolomics charts the impact of genotype-dependent methionine accumulation in Arabidopsis thaliana.
Description	Methionine (Met) is an essential amino acid for all organisms. In plants, Met also functions as a precursor of plant hormones, polyamines, and defense metabolites. The regulatory mechanism of Met biosynthesis is highly complex and, despite its great importance, remains unclear. To investigate how accumulation of Met influences metabolism as a whole in Arabidopsis, three methionine over-accumulation (mto) mutants were examined using a gas chromatography–mass spectrometry-based metabolomics approach. Multivariate statistical analyses of the three mto mutants (mto1, mto2, and mto3) revealed distinct metabolomic phenotypes. Orthogonal projection to latent structures–discriminant analysis highlighted discriminative metabolites contributing to the separation of each mutant and the corresponding control samples. Though Met accumulation in mto1 had no dramatic effect on other metabolic pathways except for the aspartate family, metabolite profiles of mto2 and mto3 indicated that several extensive pathways were affected in addition to over-accumulation of Met. The pronounced changes in metabolic pathways in both mto2 and mto3 were associated with polyamines. The findings suggest that our metabolomics approach not only can reveal the impact of Met over-accumulation on metabolism, but also may provide clues to identify crucial pathways for regulation of metabolism in plants.
Authors	Kusano M, Fukushima A, Redestig H, Kobayashi M, Otsuki H, Onouchi H, Naito S, Hirai MY, Saito K.
Reference	Amino Acids. 2010 Oct;39(4):1013-21. doi: 10.1007/s00726-010-0562-y. Epub 2010 Mar 31.
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PubMed

Sample Information

ID	S1
Title	Arabidopsis mto (Arabidopsis thaliana methionine over-accumulation)
Organism - Scientific Name	Arabidopsis thaliana
Organism - ID	NCBI taxonomy 3702
Compound - ID
Compound - Source
Preparation	The Arabidopsis mto mutants, mto1-1 (in ecotype Columbia), mto1-4 (in Columbia), mto2 [in ecotype Wassilewskija (WS)], mto3-1 (in WS), and mto3-2 (in Columbia), have been described previously (Goto et al. 2005). Seedlings were grown on Murashige and Skoog medium (Wako, Osaka, Japan, #392-00591) with 0.8% agar, 1% sucrose at pH 5.8 under 16 h light/8 h dark cycles at 22°C for 18 days. Aerial parts were harvested from 4 to 10 biological replicates of each line (see Supplementary Data 1), 6 h after the onset of the light phase. The mto mutants were backcrossed ten times for mto1-1 and mto1-4, seven times for mto2, four times for mto3-1, and seven times for mto3-2 to the WT accession (Col-0) before phenotypic and metabolite profiling analysis. Two independent alleles (mto1-1 and mto1-4) for mto1, one (mto2-1) for mto2, and two (mto3-1 and mto3-2) for mto3 were used in this study. To minimize effects derived from the different harvesting periods, we compared mutants and the control plants that were harvested in the same period [hereafter called Col0_H1 (mto1), Col0_H2 (mto2), and Col0_H3 (mto3), Supplementary Fig. 1]. For metabolite profiling, these mutants and the corresponding WT were grown under strictly controlled conditions (Kusano et al. 2007). The mto2 plants exhibit dwarf phenotypes, whereas there are no visible phenotypic changes in the mto1 and mto3 mutants when compared with the WT (Fig. 1b).
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