| MS Description
|
Frozen sample was homogenized using mill m … Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Column: DB-17ms (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 82 to 500 m/z. system (Leco), mass range: 82 to 500 m/z.
|
