SE210:/MS2
From Metabolonote
Sample Set Information
| ID | SE210 |
|---|---|
| Title | Comparison of seed metabolites among soybean varieties |
| Description | Investigation of Glycine max seed metabolites. 3 cultivers (Koitozairai, Masshoku, Misuzu) and Glycine max subsp. soja and 7 or 8 replicates data are examined. |
| Authors | Takeshi Ara 1, Yoshihiko Morishita 1, Hideyuki Suzuki 1, Kyuya Harada 2, Daisuke Shibata 1, 1: Kazusa DNA Research Institute, 2: Graduate School of Horticulture, Chiba University |
| Reference | Ara T. et al. (2009) Plant Biotechnology 26(5): 445-449 |
| Comment | 和名：小糸在来、みすず |
Analytical Method Details Information
| ID | MS2 |
|---|---|
| Title | GC-TOF-MS polar fraction method |
| Instrument | 6890N GC (Agilent Technologies); Pegasus III TOF-MS system (Leco) |
| Instrument Type | |
| Ionization | EI |
| Ion Mode | Positive |
| Description | Frozen sample was homogenized using mill mixer with liquid nitrogen. Metabolites were extracted by MeOH/H2O/CHCl3. After liquid‐liquid distribution, upper (MeOH/H2O) layer is used as polar fraction and bottom (CHCl3) layer is used as lipid fraction. Ribitol and nonadecanoic acid methyl ester were used for internal standards of polar and lipid fractions. Polar fraction was evaporated to dryness in a SpeedVac Concentrator. Metabolites were methoximated with methoxyamine hydrochloride/pyridine and then trimethylsilylated with MSTFA..GC conditions: Agilent 6890N GC (Agilent), Column: DB-17ms (Agilent), Carrier gas: He, MS conditions: Pegasus III TOF-MS system (Leco), mass range: 82 to 500 m/z. |
| Comment_of_details | Instrument Type: GC-TOF-MS |
