SE131:/S1

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Sample Set Information

ID	TSE16
Title	Integrated LC-MS/MS system for plant metabolomics
Description	Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is highly sensitive, selective, and enables extensive detection of metabolites within a sample. The result allows us to characterize comprehensive metabolite accumulation patterns without dependence on authentic standard compounds and isolation of the individual metabolites. A reference database search is essential for the structural assignment process of un-targeted MS and MS/MS data. Moreover, the characterization of unknown metabolites is challenging, since these cannot be assigned a candidate structure by using a reference database. In this case study, integrated LC-MS/MS based plant metabolomics allows us to detect several hundred metabolites in a sample; and integrated omics analyses, e.g., large-scale reverse genetics, linkage mapping, and association mapping, provides a powerful tool for candidate structure selection or rejection. We also examine emerging technology and applications for LC-MS/MS-based un-targeted plant metabolomics. These activities promote the characterization of massive extended detectable metabolites.
Authors	Yuji Sawada, Masami Yokota Hirai
Reference	Sawada and Hirai (2013) Computational and Structural Biotechnology Journal 23;4:e201301011. doi: 10.5936/csbj.201301011. eCollection 2013
Comment

ID	S1
Title	Glycine max (Soybean)
Organism - Scientific Name	Glycine max
Organism - ID	NCBI taxonomy 3847
Compound - ID
Compound - Source
Preparation	The RILs of G. max and G. soja were constructed by Hokkaido University. The RILs were distributed by the National BioResource Project (Legume Base).
Sample Preparation Details ID
Comment	RIL, recombinant inbred line